ANGPTL4, coiled-coil domain (Angiopoietin-like Protein 4, FIAF, Fasting-induced Adipose Factor, HFARP, Hepatic Fibrinogen/Angiopoietin-related Protein)

Size

£435.00

SKU:: 044531
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to the human coiled-coil domain of ANGPTL4.
Clone:: Kairos4-397G
Format:: Supplied as a liquid in PBS, pH 7.4. No preservative added.
Extra Details:: ANGPTL4 mainly expressed in endothelial cells (hypoxia-induced). Regulates angiogenesis and modulates tumorgenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA (direct or indirect): 1:500-1:5000 Immunohistochemistry: 10ug/ml Western Blot: 1:500-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.