Adiponectin, Recombinant, Rat, aa16-244, Flag-Tag (ACRP30, AdipoQ, apM1, GBP28, Adipocyte Complement Related Protein of 30kD)

Size

£544.00

SKU:: 044489
Molecular Weight:: 30
Purity:: ≥95% (SDS-PAGE). Endotoxin:: ≤0.01EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 30mM Tris-Cl, pH 8.0, 100mM sodium chloride. Reconstitute with 50ul sterile ddH2O.
Extra Details:: ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels. Rat adiponectin (aa16-244) is fused at the N-terminus to a FLAG®-tag. Molecular Weight: ~30kD (SDS-PAGE) Biological Activity: Induces the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.