Adiponectin (ACRP30, AdipoQ, apM1, GBP28, Adipocyte Complement Related Protein of 30kD)

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£397.00

SKU:: 044461
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C/-20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse adiponectin.
Clone:: MADI 04
Format:: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Extra Details:: ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels. Applications: Suitable for use in ELISA, Immunoprecipitation, Western Blot. Other applications not tested. Recommended Dilution: ELISA: direct and indirect: 1:5000-1:10,000 Immunoprecipitation: 1-5ul/sample in a final reaction volume of 500-1000ul Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice