V5 Tag

Size

£696.00

SKU:: 043700
Purity:: Purified by ammonium sulfate precipitation
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH conjugated synthetic peptide encoding the V5 tag, GKPIPNPLLGLDST.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Extra Details:: V5 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. The V5 epitope tag is derived from an epitope present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). Usually the V5 tag is used with all 14aa (GKPIPNPLLGLDST), although it has also been used with a shorter 9aa sequence (IPNPLLGLD). Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.