REST, ID (REST, NRSF, XBR, RE1-silencing transcription factor, Neural-restrictive silencer factor, X2 box repressor)

Size

£696.00

SKU:: 040963
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 542-570 in the central region of human human REST, UniProt Accession #Q13127; NP_005603.3; NP_001180437.1.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.