PC, NT (PC, Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase)

Size

£696.00

SKU:: 039714
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-82 amino acids from the N-terminal region of human PC.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq]. Applications: Suitable for use in Western Blot, Immunohistochemistry, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.