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036392

GUSB, ID (GUSB, Beta-glucuronidase, Beta-G1)

Size

£696.00

SKU:
036392
Purity:
Purified by Protein A and peptide affinity chromatography
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 335-362 in the central terminal region of human GUSB. Species Sequence Homology: porcine
Format:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:
The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220). Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Indirect Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:4000 Immunohistochemistry (FFPE): 1:100. Heat induced antigen retrieval with EDTA Buffer, pH 9.0 is recommended. Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice