ACR, ID (ACR, ACRS, Acrosin, Acrosin light chain, Acrosin heavy chain)

Size

£696.00

SKU:: 031554
Purity:: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: ACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106~135 amino acids from the Center region of human ACR.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. Applications: Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.