Synuclein, alpha (Alpha-synuclein, SNCA, Non-A beta Component of AD Amyloid, Non-A4 Component of Amyloid Precursor, NACP, PARK1)

Size

£544.00

SKU:: 031430
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length recombinant protein corresponding to human SNCA, expressed in E. coli.
Clone:: 2A7
Format:: Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol
Extra Details:: Alpha-synuclein is a member of the synuclein protein family, the other two members being β and γ-synuclein, each protein is coded for by a distinct but related gene. α-synuclein was originally isolated as a major synaptic vesicle associated protein from the electric organ of the fish Torpedo (1), and direct homologues of α-synuclein are found in all vertebrates. Later work connected α-synuclein expression with several human brain pathologies, it is a major component of the Lewy bodies of Parkinson’s disease (2). Point mutations of α-synuclein proved to be causative of some forms of familial Parkinson’s disease (3-5). One genetic cause of early onset Parkinson’s disease is duplication or triplication of one of the α-synuclein genes leading to excess production of the protein (6,7). α-synuclein is also found in the Lewy bodies of patients with diffuse Lewy body disease and inclusions in glial cells in the brains of patients with multiple system atrophy and amyotrophic lateral sclerosis. α-synuclein is normally heavily expressed in brain and appears to be localized primarily to presynaptic regions, though not with a typical synaptic vesicle distribution pattern. 031430 has also been used as a capture reagent capable of detecting endogenous α-synuclein in human plasma (8). Applications: Suitable for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunofluorescence (IC): 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice