Pdi1p (Protein Disulfide Isomerase 1p)

Size

£544.00

SKU:: 031418
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Yeast nuclear preparation
Clone:: 38H8
Format:: Supplied as a liquid, 5mM sodium azide.
Extra Details:: Pdi1p is a yeast protein disulfide isomerase. It is a major endoplasmic reticulum protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins. It also has a role in recognizing proteins targeted for ER-associated degradation (ERAD) and is a member of the protein disulfide isomerase family. Proteins in this family also can break disulfide bonds on substrate proteins. The protein is also known as Eps1p. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: For yeast cells, gives an ER staining pattern, and for this purpose should be used at a dilution of 1:500-1:5000. Western Blot: 1:5000-1:10,000 (ECL detection) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.