031410
Neuron Specific Enolase (NSE) (Not for Export EU)
Size
£544.00
- SKU:
- 031410
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Full length recombinant protein corresponding to human NSE protein, expressed in E. coli.
- Format:
- Supplied as a liquid, 5mM sodium azide.
- Extra Details:
- Human Neuron specific enolase (NSE) (EC 4.2.1.11) is a 47.14kD protein (434aa) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS). NSE catalyzes the conversion of 2-phospho-Dglycerate into phosphoenol pyruvate, and is an essential enzyme in energy metabolism in nervous tissues. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. The NSE protein runs at ~47kD on SDS-PAGE gels, and is a prominent component of brain, spinal cord and especially cortical extracts. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:500 Western Blot: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice