Neurofilament M (Neurofilament Medium Polypeptide, NF-M, Neurofilament Triplet M Protein, 160kD Neurofilament Protein, Neurofilament 3, Nefm, Nef3, Nfm) (Not for Export EU)

Size

£544.00

SKU:: 031408
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant fusion protein containing the extreme C-terminal segment of rat NF-M, aa677-845
Format:: Supplied as a liquid, 5mM sodium azide.
Extra Details:: Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable. This antibody was raised against the C-terminal region of NF-M, a region highly conserved between NF-M molecules in different species. Accordingly the antibody recognizes all mammalian and avian NF-M molecules. The name NF-M refers to the fact that this is the "middle" or "medium" protein of neurofilament triplet, the other two proteins being "low" or "light" (NF-L) and "high" or "heavy" (NF-H). NF-M is sometimes also referred to as NEF3 or Neurofilament 3. The HGNC name for this protein is NEFM. Applications: Suitable for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:2000 Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:1000-1:2500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice