Myelin Basic Protein (MBP)

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£544.00

SKU:: 031392
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified myelin basic protein isolated from bovine brain, epitope is in peptide TPPPSQGKG, amino acids 125-133 of the human 21.5kDa sequence.
Clone:: 7G7
Format:: Supplied as a liquid in PBS, 0.035% sodium azide and 50% glycerol..
Extra Details:: Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (1). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (2). In the central nervous system there are four different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 20.5, 18.5, and 17.2kD in humans. The single gene of rodents also produces 4 different proteins, but of slightly different sizes, 21.5, 18.5, 17 and 14kD. Sequence alignments of both sets of isotypes can be downloaded from here. The antibody was characterized on rat spinal cord homogenates and detected the four bands expected for that species. Since this antibody binds all four bands it is therefore a pan-specific MBP antibody, recognizing a common epitope in all four gene products. The HGNC name for this protein is MBP. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunocytochemistry/Immunofluorescence 1:1000 Immunohistochemistry 1:1000 Western Blot 1:5000-1:10000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice