Glial Fibrillary Acidic Protein (GFAP) (Not for Export EU)

Size

£544.00

SKU:: 031367
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Full-length, recombinant protein corresponding to human GFAP isotype 1, expressed in E. coli.
Format:: Supplied as a liquid, 5mM sodium azide.
Extra Details:: Glial Fibrillary Acidic Protein (GFAP) is a major CNS protein which runs on SDS-PAGE as a ~55kDa protein, usually associated with somewhat lower molecule weight bands which are alternate transcripts from the single gene. GFAP is strongly and specifically expressed in astrocytes and certain other glia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves and is also a component of neural stem cells (1-3). Astrocytes respond to many damage and disease states resulting in “astrogliosis” or the presence of a “glial response”. GFAP antibodies are widely used to see the reactive astrocytes which form part of this response, since reactive astrocytes stain much more strongly with GFAP antibodies than normal astrocytes. GFAP also forms a major component of the so-called glial scar, an astrocyte rich structure apparently forming part of the barrier to nerve fiber regeneration following damage in the central nervous system (4). Neural stem cells frequently strongly express GFAP though they lose this if they develop into neurons or oligodendrocytes. Finally, Alexander disease was recently shown to be caused by point mutations in the protein coding region of the GFAP gene (5). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes. Antibodies to GFAP are therefore very useful as a marker of glial cells in central and peripheral nerve system, as well as of developing neural stem cells. Applications: Suitable for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence/Immunocytochemistry: 1:1000-1:5000 Western Blot: 1:5000 Immunohistochemistry (paraffin): 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice