Fibrillarin (rRNA 2'-O-methyltransferase Fibrillarin, D2870, LOT3, Nucleolar Protein 1, NOP1, Nop1p, U3 Small Nucleolar RNA-associated Protein NOP1, U3 snoRNA-associated Protein NOP1, YDL014W)

Size

£544.00

SKU:: 031360
Purity:: Sterile-filtered cell culture fluid
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Yeast nuclear preparations
Clone:: 28F2
Format:: Supplied as a liquid in 5mM sodium azide.
Extra Details:: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homolog of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal ~80 amino acids contain multiple copies based on the peptide RGG, and the remaining ~240 amino acids consist of the fibrillarin domain. A fibrillarin homolog has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended b-sheets flanked by 4 a-helixes. Patients with the autoimmune disease scleroderma often have circulating autoantibodies to a 34kD protein which was subsequently found to be fibrillarin. For Nop1p/fibrillarin visualization in vertebrates, Drosophila and C. elegans and other species we recommend 031359. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:2000- Immunoprecipitation: 2-4ul for an amount of lysate derived from 5 OD600 units of yeast culture. Western Blot: For yeast protein samples, use diluted 1:10,000 (cell lysates) to 1:25,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) Western detection methods, try diluted 1:1000-1:5000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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