Lin28 (Lin-28 Homolog A, Zinc Finger CCHC Domain-containing Protein 1, CSDD1, LIN28A, ZCCHC1)

Size

£672.00

SKU:: 031089
Purity:: Purified by ammonium sulfate precipitation
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: LIN28A recombinant protein.
Clone:: 55CT58.12.1
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Lin28 is a developmentally regulated RNA binding protein (RBP). It is down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). If this degradation does not occur, then normal development of various somatic cells fails. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended Dilution: ELISA : 1:10000 Western Blot: 1:200~2000 Immunohistochemistry: 1:50~200 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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