Vav (Proto-oncogene VAV, Vav 1 Guanine Nucleotide Exchange Factor, VAV1)

Size

£652.00

SKU:: 030715
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant fragment corresponding to aa121-324 of human VAV1, expressed in E. coli.
Clone:: 2E11
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK:SAPK signaling cascade, leading to increased levels of viral transcription and replication. Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:250 Flow Cytometry: 1:200-1:400 ELISA: 1:10,000 Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.