UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1)

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£652.00

SKU:: 030714
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant fragment of human UTF1 (AA: 148-214) expressed in E. coli.
Clone:: 13B792
Format:: Supplied as a liquid in PBS, 0.05% sodium azide
Extra Details:: Undifferentiated transcription factor-1 (UTF-1) is used as marker for the undifferentiated state of pluripotent stem cells.UTF1 is a chromatin-associated protein with core histone-like properties. UTF1 further acts as a transcriptional repressor and is required for proper differentiation of pluripotent cells. UTF1 (undifferentiated embryonic cell transcription factor 1) is a 47-57kD transcriptional coactivator. It is expressed mainly in embryonic stem cells, is regulated by Oct-3/4, and promotes rapid cell proliferation. Human UTF1 is a 341aa phosphoprotein. It is rich in Proline and Arginine residues and contains a C-terminal Leu-zipper (aa286-313) that is known to interact with the metal-binding motif of ATF-2. Unlike mouse UTF1 which contains an alternate start site at Met 43, human UTF1 lacks this Methionine and shows only one isoform. Over aa44-153, human UTF1 shares 81% aa sequence identity with mouse UTF1. Applications: Western Blot: 1:500-1:2000 Flow Cytometry: 1:200-1:400 ELISA: 1:10000 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice