PIK3CA (Phosphoinositide-3-kinase, catalytic, alpha polypeptide)

Size

£652.00

SKU:: 030545
Purity:: Purified.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant fragment of human PIK3CA expressed in E. coli.
Clone:: 4F3
Format:: Supplied as a liquid in PBS with 0.05% sodium azide.
Extra Details:: Phosphatidylinositol 3-kinase is composed of an 85kD regulatory subunit and a 110kD catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. Applications: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:10,000. Immunocytochemistry/Immunofluorescence: 1:200-1:1,000. Flow cytometry: 1:200-1:400. Western Blot: 1:500-1:2,000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.