NEFL (Neurofilament light polypeptide)

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£652.00

SKU:: 030485
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant protein fragment of human NEFL expressed in E. coli.
Clone:: 13B563
Format:: Supplied as a liquid in PBS, 0.05% sodium azide, 0.5% protein stabilizer.
Extra Details:: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunofluorescence/ Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:200-1:400 ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry (Paraffin): 1:200-1:1000 Immunofluorescence (IC): 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice