030473
Myoglobin
Size
£588.00
- SKU:
- 030473
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified recombinant fragment of Myoglobin expressed in E. coli.
- Clone:
- 6H8B5, 5A2G8, 4D7H3, 3H6A7
- Format:
- Supplied as a liquid in PBS, 0.03% sodium azide.
- Extra Details:
- Myoglobin (MB), with 154-amino acid protein (about 17kD), is a member of the globin superfamily and expression of myoglobin is highest in skeletal and cardiac muscle. Functionally, myoglobin is well accepted as an O2-storage protein in muscle, capable of releasing O2 during periods of hypoxia or anoxia. Myoglobin is also thought to buffer intracellular O2 concentration when muscle activity increases and to facilitate intracellular O2 diffusion by providing a parallel path that augments simple diffusion of dissolved O2. Furthermore, myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice