Lck

Size

£659.00

SKU:: 030394
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant fragment of human Lck expressed in E. coli.
Clone:: 13B472
Format:: Supplied as a liquid, 0.03% sodium azide.
Extra Details:: Lck (lymphocyte-specific protein tyrosine kinase), with 509-amino acid protein (about 56kD), belongs to the Src non-receptor tyrosine kinases family. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Lck is expressed predominantly in T cells and is localized to the inner surface of the plasma membrane. Lck is activated after T cell stimulation and is required for T-cell proliferation and IL-2 production. Aberrant expression or activation of Lck kinase has been reported in both lymphoid and nonlymphoid malignancies. In addition, inhibition of Lck has been a target to prevent lymphocyte activation and acute rejection. Applications: Western Bloting: 1:500-1:2000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.