KSHV ORF45 (Virion phosphoprotein)

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£659.00

SKU:: 030389
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant fragment of KSHV ORF45 expressed in E. coli.
Clone:: 13B467
Format:: Supplied as a liquid, 0.03% sodium azide.
Extra Details:: Kaposi's sarcoma-associated Herpes virus (KSHV) belongs to the gamma-(2)-Herpes virus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions Applications: Western Bloting: 1:500-1:2000. Immunofluorescence: 1:200-1:1000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice