ILK (Integrin Linked Kinase)

Size

£652.00

SKU:: 030339
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant fragment of human ILK (AA: 97-244) expressed in E. coli.
Clone:: 13B417
Format:: Supplied as a liquid, 0.03% sodium azide.
Extra Details:: Integrin-linked kinase (ILK) is an ankyrin repeat-containing serine/threonine protein kinase that interacts with the cytoplasmic domain of Beta-1 and Beta-3 integrins. ILK functions with multiple binding partners to transmit signals from the extracellular matrix to actin filaments, regulating such processes as cellular attachment, cell cycle progression, and cytoskeletal organization. ILK kinase activity is induced by PI3 kinase and negatively regulated by the tumor suppressor PTEN. Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Applications: Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Flow Cytometry: 1:200-1:400 ELISA: 1:10000 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice