GPI (Glucose-6-phosphate Isomerase, Autocrine Motility Factor, AMF, Neuroleukin, NLK, Phosphoglucose Isomerase, Phosphohexose Isomerase, PHI, Sperm Antigen 36, SA-36)

Size

£659.00

SKU:: 030270
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein fragment corresponding to aa1-558 from human GPI, expressed in E. coli.
Clone:: 13B348
Format:: Supplied as a liquid, 0.03% sodium azide.
Extra Details:: Glucose-6-phosphate isomerase, or phosphoglucose isomerase is also known as GPI. It belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways and it is an enzyme that catalyzes the conversion of glucose-6-phosphate into fructose 6-phosphate in the second step of glycolysis. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in GPI are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence (IC-IF): 1:200-1:1000 ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin): 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice