EGF (Epidermal growth factor)

Size

£576.00

SKU:: 030157
Purity:: Ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant fragment of human EGF expressed in E. coli.
Clone:: 13B235
Format:: Supplied as a liquid, 0.03% sodium azide.
Extra Details:: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. ELISA: 1:10,000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.