CHIT1 (Chitinase 1, Chitotriosidase, CHI3, CHIT)

Size

£607.00

SKU:: 030094
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant protein fragment corresponding to aa22-137 from human CHIT1, expressed in E. coli.
Clone:: 13B172
Format:: Supplied as a liquid, 0.03% sodium azide.
Extra Details:: CHIT1:chitinase 1 (chitotriosidase). Chitotriosidase is a 50kD secreted glycoprotein that belongs to glycohydrolase family # 18. It is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.