HDDC3, Recombinant, Human, aa1-140 His-Tag (Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1, HD Domain-containing Protein 3, Metazoan SpoT Homolog 1, MESH1, Penta-phosphate Guanosine-3'-pyrophosphohydrolase, (ppGpp)ase, MESH1)

Size

£512.00

SKU:: 029660
Molecular Weight:: 17.9
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 40% glycerol, 0.15M sodium chloride, 1mM DTT.
Extra Details:: HDDC3, contains an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for Mn(2+) binding. Consistent with these structural data, HDDC3 efficiently catalyzes hydrolysis of guanosine 3',5'-diphosphate (ppGpp) both in vitro and in vivo. HDDC3 also suppresses SpoT-deficient lethality and RelA-induced delayed cell growth in bacteria. Source: Recombinant corresponding to aa1-140 from human HDDC3, fused to His-tag at N-terminal expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSEAAQLLE AADFAARKHR QQRRKDPEGT PYINHPIGVA RILTHEAGIT DIVVLQAALL HDTVEDTDTT LDEVELHFGA QVRRLVEEVT DDKTLPKLER KRLQVEQAPH SSPGAKLVKL ADKLYNLRDL NRCTPEVKIQ Molecular Weight: ~17.9kD (MALDI-TOF) Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.