MLK1 (Mitogen-activated protein kinase kinase kinase 9, Mixed lineage kinase 1)

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£704.00

SKU:: 029541
Application:: IHC-Fr
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic linear peptide corresponding to human MLK1 (KLH). Species sequence homology: Rat, mouse, chimpanzee and rhesus macaque (100%).
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: MLK1 (MAP3K9) is a serine–threonine kinase of the Mixed-Lineage Kinase (MLK) family capable of activating the c-Jun N-terminal Kinase (JNK)/Mitogen-Activated Protein Kinase (MAPK) pathway and regulating the other two prinicpal MAPK cascades, p38 and extracellular signal regulated kinase (ERK). Immunoblots and mass spectrometry show MLK is threonine phosphorylated in or near the activation loop. Studies show that MLKs play a role in the TGF-beta1-induced cell death mechanism. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:300 Optimal dilutions to be determined by the researcher. Positive Control: Colorectal adenocarcinoma tissue Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.