029527-BIOTIN

LAMP3 (Lysosome-associated Membrane Glycoprotein 3, LAMP-3, Lysosomal-associated Membrane Protein 3, DC-lysosome-associated Membrane Glycoprotein, DC LAMP, Protein TSC403, CD208, DCLAMP, TSC403) (Biotin)

Size

£1,086.00

SKU:: 029527-BIOTIN
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant corresponding to human LAMP-3.
Clone:: 13A205 (16H11.2)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: Lysosyme-associated membrane proteins (LAMP) are transmembrane lysosomal glycoproteins, which share a common Gly-Tyr motif as a lysosomal targeting signal. While LAMP-1 and LAMP-2 contain type 1 transmembrane domains, LAMP-3 contains a transmembrane 4 superfamily domain, tetraspanin. FceRI-mediated stimulation of basophils causes LAMP-3 to be translocated from its intracellular localization to the plasma membrane. LAMP-3 thereby serves as a reliable marker for basophil activation. LAMP-3 expression is known to be reported in human bone marrow mast cells and in leukemic human mast cell line (HMC-1), among other cell lines. LAMP3 is shown to be regulated by hypoxia in a panel of tumor cells, via the unfolded protein response (UPR). UPR is a mechanism of adaptation to endoplasmic reticulum stress and is demonstrated to contribute to hypoxic adaptation in tumors through multiple mechanisms. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): Requires HIER using sodium citrate buffer pH 6.0 Optimal dilutions to be determined by the researcher. Positive Control: MCF-7 cell lysate Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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