0004-20B
4E Binding Protein 1, non-phosphorylated (Thr46) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)
Size
£691.00
- SKU:
- 0004-20B
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding Thr46 of human 4E-BP1.
- Clone:
- 7H1(87D12)
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- 4E-BP1 (also known as PHAS-1) when bound to eIF4E, inhibits cap-dependent translation. Upon hyperphosphorylation of 4E-BP1 this interaction is disrupted and cap-dependent translation is activated (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4); while phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP-1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Flow Cytometry: 1:200 Immunofluorescence (IF-IC): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice