4-1BB Ligand (TNFSF9, CD137, CDw137, ILA, MGC2172, T-cell, 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor)

Size

£863.00

SKU:: 0004-01R
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant human 4-1BB Ligand aa71-254 (E. coli).
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: 4-1BB, also known as CD137, is a type I transmembrane protein belonging to the TNF receptor superfamily (TNFRSF9). It is expressed on T cells, NK cells, monocytes, neutrophils, dendritic cells, and eosinophils. The ligand for 4-1BB, 4-1BBL, is a TNF ligand superfamily member (TNFSF9) expressed on the surfaces of activated antigen presenting cells and T and B lymphoma cell lines. 4-1BB transduces a T cell costimulatory signal to promote survival, proliferation, cytokine production, and effector functions. On dendritic cells, 4-1BB ligation enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules. Applications: Suitable for use in RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 3-10ug/ml/10 ELISA: 0.5-1.0ug/ml Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice