66V022
AAV2 standard material (Rluc)
Cannot supply to this region.
- SKU:
- 66V022
- Application:
- ELISA, FuncS, Neutralisation
- Purity:
- > 95% by SDS-PAGE using Stain-Free Technology (Bio-Rad)
- Source:
- Produced in HEK293 based suspension cell line
- Purification:
- Iodixanol gradient centrifugation for removal of empty capsids using OptiPrep (Cat. No. 1893, PROGEN),Affinity Chromatography (POROS CaptureSelect AAVX Affinity Resin, Thermo Fisher Scientific)
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Progen
- Formulation:
- PBS + 0,001% Pluronic F68
- Extra Details:
- Our AAV2 standard material (Rluc) consists of fully assembled AAV2 capsids with a Renilla Luciferase (Rluc) reporter gene controlled by a CMV promotor. Final concentrations (total capsids and viral genome titer) are lot-specific and can be found on the CoA. The AAV2 standard material (Rluc) is provided with a titer above 1.0E+12 viral genomes/ml in a liquid formulation. The capsids can be used in various applications, including ELISA, PCR and cell-based assays, such as neutralization and transduction assays. The lot-specific titers were assigned using AAV2 ELISA (PRAAV2R or PRAAV2XP) and qPCR using Rluc primers. PROGEN provides Rluc-filled AAV standard material for the AAV serotypes 2, 5, 6, 8 and 9.
- Shipping Conditions:
- Blue Ice