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62011

Vimentin (Rind, Mr 57.000)

Cannot supply to this region.

SKU:
62011
Purity:
> 98% (determined by SDS gelelectrophoresis)
Source:
Bovine lens
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
ABP:
No Import Docs
Extra Details:
Protein standard for immunoblotting, immunization and immunoassay. Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM sodium phosphate, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). - Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841 - Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255
Shipping Conditions:
Blue Ice