62004
Keratin K18 (Rind, Mr 45.000)
Cannot supply to this region.
- SKU:
- 62004
- Additional Names:
- Cytokeratin 18
- Purity:
- > 95% (determined by SDS gelelectrophoresis)
- Source:
- Bovine liver
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Progen
- ABP:
- No Import Docs
- Extra Details:
- Protein standard for immunoblotting, immunization and immunoassays. Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). - Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841 - Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255
- Shipping Conditions:
- Blue Ice