3,6,9,12-Tetraoxatetradecane-1,14-diyl-bis-methanethiosulfonate (MTS-14-04-MTS)

Size

POA

SKU:: MBS6064868
Purity:: ~90%
Storage Conditions:: May be stored at RT for short-term only. Long-term storage is recommended at 4 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Store under inert conditions. Moisture sensitive.<br>At pH 7.5 and room temperature, MTSEA hydrolyzes with a half-life of about 15 minutes, MTSET hydrolyzes with a half-life of 10 minutes, and MTSES hydrolyzes with a half-life of 20 minutes. Routinely, one can use 2.5mM MTSEA, 1mM MTSET, or 10mM MTSES, applied for 1 to 5 minutes. (MTSET is 2.5 times as reactive with small sulfhydryl compounds as is MTSEA, and 10 times as reactive as MTSES). (Stauffer and Karlin, 1994; Karlin, personal communication).<br>For application to Xenopus Oocytes, dissolve the compounds in 115mM NaCl, 2.5mM KCl, 1.8mM MgCl2, 10mM Hepes, pH 7.5 with NaOH. MTS reagents decompose in buffer very quickly.<br>•DMSO is a good solvent for the MTS reagents which are not water soluble (i.e. the non-charged MTS reagents).
Supplier:: MyBiosource