NU-803-DEAC
DEAC-dUTP, Aminoallyl-dUTP - DEAC
Cannot supply to this region.
- SKU:
- NU-803-DEAC
- Concentration:
- 1.0-1.1 mM
- Physical State:
- Solution
- Molecular Weight:
- 766.48 g/mol (free acid)
- Purity:
- ≥95%
- Storage Conditions:
- RT, 2-8[o]C
- Supplier:
- Jena Bioscience
- Extra Details:
- DEAC-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended DEAC-dUTP/dTTP ratio for Nick Translation: 30-50% DEAC-dUTP/ 70-50% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
- Shipping Conditions:
- Blue Ice