AMCA-6-dUTP, Aminoallyl-dUTP - AMCA

Cannot supply to this region.

SKU:: NU-803-AMCA
Concentration:: 1.0-1.1 mM
Physical State:: Solution
Molecular Weight:: 851.58 g/mol (free acid)
Purity:: ≥95%
Storage Conditions:: RT, 2-8[o]C
Supplier:: Jena Bioscience
Extra Details:: AMCA-6-dUTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended AMCA-6-dUTP/dTTP ratio for PCR and Nick Translation: 20-30% AMCA-6-dUTP/ 80-70% dTTP (PCR), 30-50% AMCA-6-dUTP/ 70-50% dTTP (Nick Translation) Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.