CLK-087
5-Ethynyl-cytidine (5-EC)
Cannot supply to this region.
- SKU:
- CLK-087
- Physical State:
- Solid
- Molecular Weight:
- 267.24 g/mol
- Purity:
- ≥99%
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles. Desiccated.
- Supplier:
- Jena Bioscience
- CAS Number:
- 65223-78-1
- Extra Details:
- Ethynyl-labeled cytidine (5-EC) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EC is cell permeable and incorporates into nascent RNA instead of its natural analog cytidine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1]. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
- Shipping Conditions:
- Ambient