Citrate Fluorometric Assay Kit

Size

£182.70

Was £203.00

SKU:: E-BC-F153
Physical State:: Plasma, Serum, Tissue
Storage Conditions:: -20[o]C Avoid freeze/thaw cycles. Protect from light.
Supplier:: Elabscience
Format:: Fluorometric method
Extra Details:: Citrate is widely present in animal blood, tissues, and plant fruits. In living organisms, citrate is typically synthesized from oxaloacetate and acetyl-CoA via citrate synthase. As an intermediate metabolite in the tricarboxylic acid (TCA) cycle, CA can be rapidly utilized to generate ATP under stress conditions, thereby enhancing the organism's resistance to stress. Citrate is converted into oxaloacetate under the catalysis of citrate lyase. Oxaloacetate is subsequently transformed into hydrogen peroxide (H2O2) via multiple enzymatic reactions. The generated H2O2 reacts with the fluorescent probe ADHP to produce a fluorescent substance. The fluorescence intensity can be measured to calculate the citrate content in the sample.