Prdm9 Polyclonal Antibody

Size

£195.30

Was £217.00

SKU:: E-AB-92241
Additional Names:: BC012016, Dsbc, Dsbc1, G1-419-29, Meise, Meisetz, PRDM9-B, Rcr1, re, repro7,Rc
Application:: WB
Concentration:: 1 mg/ml
Purification:: Affinity Purified
Storage Conditions:: -20[o]C Avoid freeze/thaw cycles.
Supplier:: Elabscience
Host:: Rabbit
Reactivities:: Mouse, Rat
Buffer:: Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Immunogen:: Recombinant fusion protein of mouse Prdm9
Uniprot:: Q96EQ9
Synonyms:: [histone H3]-lysine36 N-trimethyltransferase PRDM9;[histone H3]-lysine4 N-trimethyltransferase PRDM9;[histone H3]-lysine9 N-trimethyltransferase PRDM9;[histone H4]-lysine20 N-methyltransferase PRDM9;[histone H4]-N-methyl-L-lysine20 N-methyltransferase PRDM9;BC012016;Dsbc;Dsbc1;G1-419-29;histone H3 lysine-4 methyltransferase;histone-lysine N-methyltransferase PRDM9;hybrid sterility protein 1;meiosis-induced factor containing a PR/SET domain and zinc-finger motif;Meise;Meisetz;PR domain zinc finger protein 9;PR domain-containing protein 9;PRDM9-B;protein-lysine N-methyltransferase PRDM9;Rc;Rcr1;re;repro7;VPR domain containing 9
Extra Details:: Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4 and 'Lys-36' (H3K36 of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3 and trimethylated 'Lys-36' (H3K36me3 histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination. Also can methylate all four core histones with H3 being the best substrate and the most highly modified. Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate. During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity. Thereby promotes double-stranded breaks (DSB formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression.

Shipping Conditions:: Blue Ice