D-AB-10421L
CD146/MCAM Polyclonal Antibody
Size
£120.60
Was £134.00
- SKU:
- D-AB-10421L
- Additional Names:
- MCAM, MUC18,CD146
- Application:
- WB
- Concentration:
- 1 mg/ml
- Purification:
- Affinity Purified
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles.
- Supplier:
- Elabscience
- Host:
- Rabbit
- Reactivities:
- Human
- Buffer:
- PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
- Immunogen:
- Recombinant Human CD146/MCAM protein expressed by E.coli
- Uniprot:
- P43121
- Synonyms:
- CD146;cell surface glycoprotein MUC18;cell surface glycoprotein P1H12;Gicerin;HEMCAM;melanoma adhesion molecule;Melanoma cell adhesion molecule;melanoma-associated antigen A32;melanoma-associated antigen MUC18;MelCAM;METCAM;MUC18;S-endo 1 endothelial-associated antigen
- Extra Details:
- CD146,also known as melanoma cell adhesion molecule (MCAM) or MUC18,originally identified as a biomarker of melanoma progression,is a transmembrane glycoprotein of 113-130 kDa,belonging to the immunoglobulin (Ig) superfamily. Structurally,it consists of five Ig domains,a transmembrane domain,and a cytoplasmic region. In normal adult tissue,CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis,and has been used as marker of circulating endothelium cells (CECs). In addition to the membrane-anchored form of CD146,a soluble form of CD146 (sCD146,105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells. This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F,which removes oligosaccharides from N-linked glycoproteins,led to a down-shift of the detected band.
- Shipping Conditions:
- Blue Ice