AN302055L
Recombinant RNF40 Monoclonal Antibody
Size
£252.90
Was £281.00
- SKU:
- AN302055L
- Additional Names:
- RBP,RNF,KIAA,RNF40,BRE1B,RBP95,STARING,KIAA0661
- Application:
- WB
- Concentration:
- 1 mg/ml
- Purification:
- Protein A Purified
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles.
- Supplier:
- Elabscience
- Host:
- Rabbit
- Reactivities:
- Human
- Buffer:
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
- Immunogen:
- Peptide. This information is proprietary to PTMab
- Clone:
- A775
- Uniprot:
- O75150
- Synonyms:
- 95 kDa retinoblastoma protein binding protein;95 kDa retinoblastoma-associated protein;BRE1 E3 ubiquitin ligase homolog B;BRE1-B;BRE1B;E3 ubiquitin-protein ligase BRE1B;Rb-associated protein;RBP95;RING finger protein 40;ring finger protein 40, E3 ubiquitin protein ligase;RING-type E3 ubiquitin transferase BRE1B;STARING
- Extra Details:
- In mammalian cells, the significance of histone H2B ubiquitination in chromatin epigenetics came from the identification of the budding yeast protein Bre1. Together with the ubiquitin-conjugating enzyme Rad6, Bre1 serves as the E3 ligase in the monoubiquitination of the yeast histone H2B within transcribed regions of chromatin. Subsequently, the mammalian orthologs of yeast Bre1, RNF20 and RNF40, were identified. These two proteins form a tight heterodimer that acts as the major E3 ligase responsible for histone H2B monoubiquitination at Lys120 in mammalian cells, a modification linked to RNA Pol II-dependent transcription elongation in undamaged cells. Researchers have shown that DNA double-strand breaks (DSBs) are also capable of inducing monoubiquitination of H2B. This process depends upon the recruitment to DSB sites, as well as ATM-dependent phosphorylation of the RNF20-RNF40 heterodimer, thus highlighting a role for this E3 ligase in DSB repair pathways. Indeed, investigators have shown that loss of RNF20-RNF40 function promotes replication stress and chromosomal instability, which may constitute an early step in malignant transformation that precedes cell invasion.
- Shipping Conditions:
- Blue Ice