PE Anti-Mouse Ly-49C/I Antibody[5E6]

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£81.00

Was £90.00

SKU:: AN00659D
Additional Names:: Ly49C,Ly49I
Application:: Flow Cytometry
Concentration:: 5 ul/test
Storage Conditions:: 2-8[o]C Do not freeze. Protect from light.
Supplier:: Elabscience
Host:: Mouse
Reactivities:: Mouse
Buffer:: PBS
Immunogen:: Ly-49C/I
Uniprot:: Q2TJJ8, Q64329
Synonyms:: 5E6;killer cell lectin-like receptor 3;killer cell lectin-like receptor subfamily A member 9 transcript variant 1-L;killer cell lectin-like receptor subfamily A member 9 transcript variant 1-S;killer cell lectin-like receptor subfamily A, member 9;Ly4;Ly49;Ly49c;Ly49I;Ly49I receptor;LY49I1;lymphocyte antigen 49c;natural killer cell-associated antigen 2;Nk;Nk-;Nk-2;NK-2.;NK-2.1;Nk2;Nk2.;Nk2.1;T-cell surface glycoprotein Ly-49C
Extra Details:: The 5E6 (also known as clone SW5E6) antibody reacts with Ly-49C[BALB], Ly-49C[B6], Ly-49C[NZB], and Ly-49I[B6], inhibitory receptors which are expressed on subsets of natural killer (NK) cells and NK-1.1+ (or DX5+) T lymphocytes (NK-T cells) in all strains tested except C57BR and RIII, on a population of memory CD8+ T lymphocytes and NK1.1+ G GammaD Delta T cells in C57BL/6 mice, and on a distinct subset of B-1 cells of BALB/c and C57BL/6 mice. The proportion of NK T cells expressing Ly-49C/I is higher (2-5 fold) in thymus than in liver (immature and mature NK T cells, respectively), and there is evidence that the down-regulation of Ly-49 receptor expression is necessary for normal NK T-cell development. Most NK cells express a single allele of Ly-49C, although occasionally they may express more than one allele. The Ly-49 family of NK-cell receptors are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains (CRD) that bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. The 5E6 antibody is specific for the Ly-49C CRD. The Ly-49C[BALB] and Ly-49C[B6] alloantigens bind to MHC class I antigens of the b, d, k, and s haplotypes, and the 5E6 antibody blocks this binding. Binding of Ly-49C[BALB]- and Ly-49C[B6]- expressing transfectants to lymphoblasts of H-2[f], H-2[q], H-2[r], and H-2[v] strains has also been detected. Ly-49I[B6] transfectants bind H-2[r] lymphoblasts and bind much more weakly to the b, d, k, q, s, and v haplotypes. The levels of the Ly-49 inhibitory receptors are down-regulated by their ligands in vivo, and the various levels of expression of an Ly-49 inhibitory receptor may affect the specificity of NK cells. Ly-49C is specifically downregulated in the presence of H-2K[b] class I molecules (one of the Ly-49C ligands). Ly-49C[+] and/or Ly-49I[+] cells mediate allogeneic and hybrid resistance to H-2d bone marrow transplantation. In vitro and in vivo studies suggest that the Ly-49C and/or Ly-49I receptors mediate negative regulation of NK-cell cytolytic activity via tyrosine phosphorylation of their ITIMs (Immunoreceptor Tyrosine-based Inhibitory Motifs).

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