Anti-Prion Protein Antibody

Size

£352.00

SKU:: PRN
Additional Names:: Major prion protein, PrP, ASCR, PrP27-30, PrP33-35C, CD antigen CD230
Application:: IHC, WB, ICC
Concentration:: 10 mg/ml
Physical State:: Liquid
Molecular Weight:: 27 kDa
Species Reactivity:: Human
Purification:: Chickens were immunized with a recombinant fragment of human PRN protein emulsified in Freund's adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using the PRN protein conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 ug/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.
Storage Conditions:: -20[o]C Avoid freeze/thaw cycles. Protect from light.
Supplier:: Aves Labs
Host:: Chicken/Avian
Reactivities:: Human, Mouse, Rat
Buffer:: Sodium phosphate (10 mM
Immunogen:: Recombinant fragment of human prion
Formulation:: Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent.
Format:: Mixture of IgY fraction and affinity-purified antibodies
Uniprot:: P04156
Synonyms:: alternative prion protein;AltPrP;ASCR;CD230;CD230 antigen;CJD;GSS;KURU;major prion protein;p27-30;prion-related protein;PRIP;PrP;PrP27-30;PrP33-35C;PrPc
Extra Details:: Antibodies were prepared by injecting laying hens with purified recombinant human PRN protein. After a series of boosts, eggs were collected from hyperimmunized aniamsl and the IgY fraction prepared. Affinity purified antibodies were then isolated from the IgY fraction using a PRN protein column. Finally, affinity purified antibodies were mixed with the IgY fraction to make the final product. These antibodies have been validated with human, mouse and rat tissues., Aves Labs

Shipping Conditions:: Blue Ice