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PAP

Anti-Prostatic Acid Phosphatase (PAP) Antibody

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£352.00

SKU:
PAP
Additional Names:
Prostatic acid phosphatase, PAP, EC 3.1.3.2., 5'-nucleotidase, 5'-NT, EC 3.1.3.5, Ecto-5'-nucleotidase, Thiamine monophosphatase, TMPase, [Cleaved into: PAPf39]
Application:
IHC, WB, ICC
Concentration:
10 mg/ml
Physical State:
Liquid
Molecular Weight:
45 kDa
Species Reactivity:
Mouse
Purification:
Chickens were immunized with recombinant mouse Prostatic Acid Phosphatase protein emulsified in Freund's adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using the PAP protein conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 ug/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles. Protect from light.
Supplier:
Aves Labs
Host:
Chicken/Avian
Reactivities:
Human, Mouse, Rat
Buffer:
Sodium phosphate (10 mM
Immunogen:
Recombinant mouse PAP expressed in Escherichia coli
Formulation:
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent.
Format:
Mixture of IgY fraction and affinity-purified antibodies
Uniprot:
P15309
Synonyms:
5'-NT;5'-nucleotidase;acid phosphatase, prostate;ACP-3;ACPP;ecto-5'-nucleotidase;prostatic acid phosphatase;prostatic acid phosphotase;protein tyrosine phosphatase ACP3;thiamine monophosphatase;TM-PAP;TMPase
Extra Details:
Recombinant mouse PAP protein was expressed in bacteria. Antibodies against this protein were prepared by injecting purified recombinant PAP into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been validated with human, mouse and rat tissues., Aves Labs
Shipping Conditions:
Blue Ice