NES
Anti-Nestin Antibody
Size
£563.00
- SKU:
- NES
- Additional Names:
- Nestin
- Application:
- IHC, WB, ICC
- Concentration:
- 300 ug/ml
- Physical State:
- Liquid
- Molecular Weight:
- 250 kDa
- Species Reactivity:
- Mouse
- Purification:
- Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 ug/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
- Storage Conditions:
- 2-8[o]C Protect from light.
- Supplier:
- Aves Labs
- Host:
- Chicken/Avian
- Reactivities:
- Mouse, Rat
- Buffer:
- Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS,"
- Immunogen:
- Synthetic peptides
- Formulation:
- Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
- Format:
- Affinity-Purified IgY
- Uniprot:
- Q6P5H2
- Synonyms:
- AA166324;C78523;ESTM4;ESTM46;Ifa;Ifaprc2;intermediate filament protein;intermediate filament-associated protein RC2;Mar;Marc2;nestin;RC;RC2
- Extra Details:
- Three antipeptide antibodies were generated in chickens against a sequence in mouse (NP_057910) gene product. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues., Aves Labs
- Shipping Conditions:
- Blue Ice