ACT-1040
Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody
Size
£364.00
- SKU:
- ACT-1040
- Application:
- WB
- Concentration:
- 10 mg/ml
- Physical State:
- Liquid
- Molecular Weight:
- 42 kDa
- Species Reactivity:
- Rabbit
- Purification:
- Chickens were immunized with purified actin from rabbit muscle emulsified in Freund's adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using actin conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 ug/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles. Protect from light.
- Supplier:
- Aves Labs
- Host:
- Chicken/Avian
- Reactivities:
- Human, Mouse, Rat
- Buffer:
- Sodium phosphate (10 mM
- Immunogen:
- Actin purified from Rabbit muscle
- Formulation:
- Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent.
- Format:
- Mixture of IgY fraction and affinity-purified antibodies
- Uniprot:
- P63267
- Synonyms:
- ACT;ACTA3;ACTE;actin-like protein;actin, gamma 2, smooth muscle, enteric;actin, gamma-enteric smooth muscle;ACTL3;ACTSG;alpha-actin-3;Gamma-2-actin;MMIHS5;Smooth muscle gamma-actin;VSCM;VSCM1
- Extra Details:
- Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes., Aves Labs
- Shipping Conditions:
- Blue Ice