YXX26701

Lba Cas12a (Cpf1) Nuclease

Size

£177.00

SKU:: YXX26701
Additional Names:: CRISPR-associatedendonucleaseCas12a,CRISPR-associatedendonucleaseCpf1,LbaCas12a(Cpf1),LbaCas12a,Cpf1,LbCas12a
Physical State:: Liquid
Purity:: >90% as determined by sds-page.
Storage Conditions:: -20[o]C Avoid freeze/thaw cycles.
Supplier:: AntibodySystems
Species:: Bacteria
Extra Details:: LbaCas12anucleaseisderivedfromLachnospiraceaebacteriumND2006andisasite-specificDNAendonucleasemediatedbyasingle-guideRNA(gRNA)withalengthof41-44nucleotides.InthepresenceofaPAM(TTTN)sequenceonthetargetdouble-strandedDNA,LbCas12aspecificallycleavesthetargetdsDNA,resultingindouble-strandbreaksandthegenerationofstickyends.Incontrast,thespecificcleavageofsingle-strandedDNAtargetsbyLbCas12adoesnotrequireaPAMsequence.\r\n LbaCas12acontainssimianvirus40(SV40)Tantigennuclearlocalizationsequences(NLS)atboththeN-terminusandC-terminus.WhenCas12aisexpressedwiththeseNLSsequences,theCas12a-RNPcomplexcanrapidlylocalizetothenucleusuponcellularentry.\r\n Additionally,bothdouble-strandedandsingle-strandedDNAtargetscanactivatethetrans-cleavageactivityofLbCas12a.Specifically,uponformationofaternarycomplexamongtheLbCas12aenzyme,crRNA,andtargetDNA,theenzymebecomesactivatedandexhibitsnon-specifictrans-cleavageactivity,degradinganysingle-strandedDNA(ssDNA)presentinthesystem.Consequently,LbCas12acannotonlybeusedforspecificcleavageofdsDNAinvitrobutalsoenablesrapiddetectionoftargetnucleicacids.