Click-&-Go[R] Plus 647 OPP
Product Sizes
1 kit
CCT-1496-1KIT
About this Product
- SKU:
- CCT-1496
- Application:
- Conjugation/Labeling
- Extra Details:
- Click-&-Go® Plus 647 OPP Protein Synthesis Assay Kit is a non-radioactive method for measuring protein synthesis that employs the cell-permeable, alkyne-containing, puromycin analog O-Propargyl-puromycin (OPP). OPP (O-propargyl-puromycin) is an analog of puromycin that contains a terminal alkyne group, enters the acceptor site of ribosomes and incorporates into the C-terminus of translating polypeptide chains, thereby stopping translation. These truncated C-terminal alkyne-labeled proteins are then subsequently detected via copper-catalyzed click reaction using far-red fluorescent AZDye 647 Azide Plus. Unlike HPG or AHA labeling reagents OPP is not an amino acid analog; thus, OPP can be added directly to cells in complete media (i.e., methionine-containing) or used to detect in vivo protein synthesis. The kit contains all of the components needed to label and detect the incorporated OPP in newly translated proteins in samples of adherent cells. The kit includes sufficient reagents for the labeling of 25 18 mm x 18 mm coverslips using 1 mL of reaction buffer per test.
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 2-8[o]C
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Fluorophores
- Manufacturer's Data Sheet:Click-Go-Plus-647-OPP
